Microbiology Lab Identification Of Unknown Bacteria Quizlet

The oxidase test is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain. Second lab in a series on identifying unknown bacteria. It will also show the results of the activity of those enzymes. IDENTIFICATION OF UNKNOWN BACTERIA LAB REPORT Grace K. Microbiology Lab Quiz. Many species of non-fermenting Gram-negative bacilli (non-fermenters) are important opportunistic and nosocomial pathogens. ALL of the following were performed on the unknown Phenol Red Broth, Methyl Red, Voges Proskauer, Citrate, Nitrate Reduction, Gelatinase, Urease, SIM. ), carbon source utilization (Microlog; Biolog, Inc. Fast Lab Results, Quality Data! We Identify Unknowns! Customize Your Lab Reports! EMSL Analytical, Inc. Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology text–and now features brief clinical applications for each experiment, MasteringMicrobiology ® quizzes that correspond to each experiment, and a new experiment on hand washing. Essay Microbiology Unknown Bacteria. Unlike a virus, bacteria are easily traceable by simple staining methods. MR (Methyl Red) Nitrate Reduction. One example of an extreme survival strategy employed by certain low G+C Gram-positive bacteria is the formation of endospores. Examples of Antibiotic Sensitivity Testing Methods Module Key Points References and Suggested Readings Public Health. Growth rate vs pH for three environmental classes of procaryotes. He owes many of his discoveries to well-chosen collaborations. Featured Quizzes. Exercises for the Microbiology Laboratory, 4th ed. Bugs In The News, maintained by John C. Biochemical tests for identification of bacterial pathogens Overview of a medical microbiology laboratory - Duration: 7:55. Bacteria which. The results of these tests on the suspected microorganism are then compared to known results for that organism to confirm its identification. 13 Viruses, Viroids, and Prions. The first procedure that was done was a gram stain followed by a streak of the unknown on a TTS plate in order to determine the gram reaction and observe the colony. aureus is with some frequency found as normal human flora in the anterior nares (nostrils). For EU (identification of a bacterial species belonging to the Enterobacteriaceae family) and OU (identification of a non-Enterobacteriaceae bacterial species), we use the conventional microbiological diagnostic techniques to identify the species. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Historically, microbiologists have mostly relied on culturing and single-gene diversity surveys to understand Archaea in nature. Microbes can make a big difference. Microbiology Pictures. I need help identifying an unknown organism. Enteric Unknown Report Microbiology UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. An unknown labeled 123 was given out by the lab instructor. The techniques used were applied to identify an unknown bacterium. MATERIALS AND METHODS. Identifying an unknown is a fascinating process. Spring 2014. We use phenotype analysis to identify bacteria. Aseptic techniques such as sterilizing the loop and needle and using the zone of aseptis were used during inoculation. Laboratory Exercises in Microbiology, 5th ed. Unknown C was determined to be a Gram- negative rod. Then the results were compared with the chart of known characteristics. After having collected all the data, the list of bacteria was reduced to only two choices; the Proteus pneumonia and the Klebsiella pneumoniae. View Lab Report - BIO210_Lab10_Mod07_UnknownIdentification. Students are introduced to the Acid-fast and Endospore stains. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. Find many great new & used options and get the best deals for Microbiological Applications : A Laboratory Manual in General Microbiology, Complete Version by Harold J. Culture media in microbiology is a media where microorganisms like bacteria from a sample is grown into colony for the identification of particular organism. Microbiology 210 Lab Final Unknown Report Section#006/ Radha. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Bacteria _ Gram Reaction. This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor. Overview of a medical microbiology laboratory - Duration: 7:55. The widespread use of this technology has led to the development of automated systems and databases for the identification of non-culturable organisms and medically important bacteria ( 4 , 5 , 16 ). This kit is one that I plan to try this year in order to help run some tests to identify what the bacterial colonies might be. Microbiology Lab Midterm. In this particular study an unknown was given by the professor. The unknown bacteria were also used in at least two reactions per primer to assess the reproducibility of the method. I Gram stained it and found Gram+ cocci, and Gram- rods. MacConkey is usually fairly decent, especially if you want to test the lactose fermentation thing. Microbiology - Practical Practice Questions Topics: see Study Objectives listed at beginning of LAB MANUAL for more specific information about WHAT EXERCISES will be covered for each practical. Unlike a virus, bacteria are easily traceable by simple staining methods. carbohydrate utilization tests (sugar test). Bacteria are ubiquitous, and live within the human gut, and in every corner of our environment. A good definitive test for Gram + cocci is the catalase test. Bacteria that are gram negative will appear pink while those that are gram positive will appear purplish. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. In the medical field bacteria and infections of different kinds are the core of the practice. Step 2: Identification. They can be dangerous, such as when they cause infection, or beneficial, as in the process of fermentation (such as in wine) and that of decomposition. Identification of gram-negative aerobes relies on whether or not. This is not an all inclusive chart and focuses mainly. The following study was performed using all of the methods used in general microbiology laboratory class. The broth contains two pH buffers, urea, a very small amount of nutrients for the bacteria, and the pH indicator phenol red. Bacteria have been identified that have beneficial qualities such as necessary organic matter decomposition and use in food, chemical and antibiotic drug production. The critical nature of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician/advanced practice provider and the microbiologists who provide enormous value to the healthcare team. Materials and Methods. By going through the steps to identify the microorganism, students began to comprehend infectious disease. The techniques used were applied to identify an unknown bacterium. The organisms selected include types you have studied this semester and some new genera. Nutrient agar is always a safe choice. The unknown bacteria were also used in at least two reactions per primer to assess the reproducibility of the method. The 7th Edition can be found online at:. pertussis and B. Microbiology. NAME OF EXERCISE. The results of these tests on the suspected microorganism are then compared to known results for that organism to confirm its identification. The following tests were performed on the gram-­‐positive bacteria: 1. ), and 16S rRNA gene sequence. Identification of an Unknown Bacterium and Writing Up a Report Start studying Microbiology Dichotomous Key Quiz. Introduction Bacteria in nature do not exist as pure cultures like the ones that we have used in lab. The study of microorganisms is greatly facilitated if we are able to culture them, that is, to keep reproducing populations alive under laboratory conditions. Antibiotic Sensitivity. See more ideas about Types of crystals, Medical laboratory science, Microbiology. Materials and Methods A mixed culture of two unknown bacteria was provided by the instructor. This interactive, modular lab explores the techniques used to identify different types of bacteria based on their DNA sequences. This method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in their cell walls. The emergence of novel plasmid-mediated resistance genes constitutes a great public concern. of Bacillus megaterium. Microbiology. Microscopy to identify cell shape or appearance of spores. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. Therefore, when the proper safety precautions are taken, colonies of microorganisms can be safely. Identification of unknown bacteria by. Although most bacteria are produced with single microscopic cells, they are not as easy as previously believed. Layman searches for pesky unknown bacteria. McGraw-Hill Higher Education, New York, NY, USA. After a series of differential tests, a chart of bacteria was referenced to discover the unknown bacteria was E. The structural differences with respect to shape, size and arrangement of bacteria only help in the process of identification, because there are many species of bacteria having similar shape, size and arrangement. Broad-host-range self-transferable plasmids are known to facilitate bacterial adaptation by spreading genes between phylogenetically distinct hosts. If you are still unsure of the identity of your unknown, inoculate other tests. Do a Gram stain on the unknown (see Lab 6). NAME OF EXERCISE. Carbohydrate Utilization. Step 2: Identification. Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology text–and now features brief clinical applications for each experiment, MasteringMicrobiology ® quizzes that correspond to each experiment, and a new experiment on hand washing. Apr 15, 2019 - Explore kbayele's board "microbiology" on Pinterest. Short Answer Questions. A microbiology laboratory, or lab, is the primary place that a working microbiologist not in the field can be found. Students are introduced to the Acid-fast and Endospore stains. Microbiology 20 Biochemical Unknown – Spring 2009 (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May 14th Biochemical tests in microbiology lab. lab 3: obtaining pure cultures from a mixed population; lab 4: enumeration of microorganisms; lab 5: direct stain and indirect stain; lab 6: gram stain and capsule stain; lab 7: endospore stain and bacterial motility; lab 8: using biochemical testing to identify bacteria; lab 9: fungi, part 1 - the yeasts. The Unknown #121 was a combination of two different bacterium, one Gram positive and one Gram negative. Clinical microbiology focuses on the isolation and characterization of infectious organisms so they can be managed and treated in patients. , Newark, Del. The microscopes have varied applications and modifications that contribute to their usefulness. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. All of these processes are increasingly becoming partnered more intimately with informatics. The techniques used were applied to identify an unknown bacterium. Identification of most species of non-fermenters by phenotypic characteristics can be difficult. Microbiology laboratories use published methods to identify bacteria. The appearance of the discrete masses of growth or colonies that can be grown from isolated bacteria on the surface of the solid medium (nutrient agar) can be used to study the size of the colonies (diameter in mm), their outline (whether circular, entire, indented or wavy or. Use standard laboratory procedures, like cell staining, culturing and DNA sequencing to further narrow down your identification. The 7th Edition can be found online at:. Specialized media (MacConkey's & Mannitol Salt) are inoculated with unknown to assist in identification of unknown bacteria using a dichotomous key. Identification of Mixed Unknown Bacterial Culture I Identification of Mixed Unknown Bacterial Culture I (found on the Microbiology Web site - a flow-chart that guides you through a series of tests to a [Filename: bisc300-lab_ident_unknown. Microbiology 20 Biochemical Unknown – Spring 2009 (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May 14th Biochemical tests in microbiology lab. Pommerville J. INTRODUCTION: Microorganisms make up not only sixty percent of the earth's biomass, but also generate over half of the oxygen one breaths each day (Fascinating Facts). MATERIALS AND METHODS:. Many pathogenic bacteria exhibit a relatively narrow range of pH over which they will grow. The topic of "BIOL 260-Microbiology (Bacterial Unknown Lab report)" is quite popular among the tasks in university. We work with public health partners in Canada and abroad to prevent the spread of infectious diseases. Essay Microbiology Unknown Bacteria. MATERIALS AND METHODS. Bugs In The News, maintained by John C. THE RELATIONSHIP OF IDENTIFICATION TO BACTERIAL CLASSIFICATION AND NOMENCLATURE. During the lab exercise the only problem encountered was the process in isolating the bacteria mixture. DNA Analysis. The unknown bacteria will be one of the following: Enterococcus faecalis, Staphylococcus saprophyticus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Salmonella [I assume typhimurium], or Shigella [either flexneri or sonnei, we used both in our lab during the semester]. Just what ARE these little bugs in the incubator ???? Student attempts identification of unknown bacteria in hopes. We have seen that identifying bacteria isolated in the clinical laboratory by sequence instead of phenotype can improve clinical microbiology by better identifying poorly described, rarely isolated, or biochemically aberrant strains. Oxidase Test. Print a copy of the rubric from the links above. This medium contains a small amount of KNO3 (potassium nitrate). The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns. Kennedy (1) unless otherwise noted. By going through the steps to identify the microorganism, students began to comprehend infectious disease. The unknown bacteria were also used in at least two reactions per primer to assess the reproducibility of the method. Spring 2014. Layman searches for pesky unknown bacteria. Difference Between. Microbiology Unknown Project - 2014. Identification of Mixed Unknown Bacterial Culture I Identification of Mixed Unknown Bacterial Culture I (found on the Microbiology Web site - a flow-chart that guides you through a series of tests to a [Filename: bisc300-lab_ident_unknown. INTRODUCTION The discipline of microbiology has many applications in science and medicine. Before going over the tests to identify an unknown bacterial species, we should remember some bases of manipulating bacteria. During a bacteria culture test, a sample will be taken from your blood, urine, skin, or other part of your body. Bacteria can. Unknown: Gram Stain and Prepare Reserve and Working Stocks: Food Microbiology: Preparation of Wine: T: Nov. Brown of the University of Kansas Department of Microbiology, is a set of articles on newsworthy bacteria and other microbes. • A colony is defined as a visible mass of microorganisms all originating from a single mother cell. In microbiology, the basic taxonomic unit is the species, and groups of related species are placed in the same genus. "The Good, the Bad, and the Deadly". Microbiology - Practical Practice Questions Topics: see Study Objectives listed at beginning of LAB MANUAL for more specific information about WHAT EXERCISES will be covered for each practical. The following study was performed using all of the methods used in general microbiology laboratory class. Tyler Wolfangel. Phet Building Molecules Virtual Lab Packet Answers. com Microbiology Lab Report 3 Introduction: This study shows the importance of identifying unknown microorganisms by applying all the methods and techniques that were studied in the microbiology course throughout the semester. In 1872, Ferdinand Cohn, a contemporary. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Essay Microbiology Unknown Bacteria. The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. ADVERTISEMENTS: The following points highlight the seven steps for identification of bacteria isolated from a specimen. Spring 2014 INTRODUCTION The purpose of this study was multifaceted: First, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment. The MLG contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the. Specialist and reference microbiology: laboratory tests and services Isolate identification (unknown, atypical, fastidious, emerging bacteria) Gram-negative bacteria non fermenter and. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 13 Viruses, Viroids, and Prions. In later labs we will use a wide variety of special purpose differential media frequently used in the clinical laboratory to identify specific pathogenic. We work with public health partners in Canada and abroad to prevent the spread of infectious diseases. Antibiotic Sensitivity. An unknown test tube labeled 120 was given out by the lab instructor. Samples of foods will be available in the laboratory up to a week before your scheduled lab period. The techniques used were applied to identify an unknown bacterium. Materials and Methods. The students will then use the Gram staining and streak plate methods to determine the end result of the bacteria. (note: All bacteria that are oxidase positive are aerobic, and can use oxygen as a terminal electron acceptor in respiration. Identification of an Unknown Bacterium and Writing Up a Report A standard part of nearly all lab courses in introductory microbiology is an activity wherein the student must use everything that has been learned in the course to identify and unknown bacterial culture. In the medical field bacteria and infections of different kinds are the core of the practice. The following test below shows my journey towards finding my unknown. Short Answer Questions. The coagulase-negative staphylococci (CNS) are now known to comprise over 30 other species. luteus is a Gram positive cocci (as seen by a gram stain). 2012 – Unknown organism was streaked from a mixed broth onto both TSA and BHA plates in an attempt to isolate pure colonies for testing. Paul, Laboratory manual and work book in Microbiology Applications to patient care, seventh edition, McGraw-Hill Higher Education Prescott M. In this lab, students identify an unknown bacteria using a biochemical method and a molecular method. Microbiology 210 Lab Final Unknown Report Section#006/ Radha. View Lab Report - BIO210_Lab10_Mod07_UnknownIdentification. pdf] - Read File Online - Report Abuse. Spring 2013. Dec 16, 2019 - Qualified Unknown Bacteria Identification Bacteria Biochemical Test Flow Chart Microbiology Lab Unknown Bacteria Flow Chart MicrobiologyBack To 18 Memorable Unknown Bacteria IdentificationExpert Unknown Bacteria Identification…. Main approaches to Microbial identification. General Microbiology Concordia University, Irvine February 24, 2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop. From heart valve Sound and Fury in the Microbiology Lab. Materials and Methods. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Materials and Methods A mixed culture of two unknown bacteria was provided by the instructor. This is not an all inclusive chart and focuses mainly. The broth contains two pH buffers, urea, a very small amount of nutrients for the bacteria, and the pH indicator phenol red. I went through a wet lab in school, and starting off with gram+ or gram- narrows down the field alot. The goal of. Their genetic sequences indicated that each of these Domains arose separately from an ancestor. mirabilis, because the organism has a tendency to swarm across the agar (because of it’s high motility), so that’s cool to see. MATERIALS AND METHODS. , University of Oregon Science Education in Rural Schools Research Interests: Ecology, evolution and systems biology of Pelagibacterales (SAR11). Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. Make sure you review the instructions before you do the Gram stain. If you are still unsure of the identity of your unknown, inoculate other tests. 29 April 2014. We also offer a range of biochemical reagents, known for the identification of specific metabolisms and to differentiate between bacteria. (note: All bacteria that are oxidase positive are aerobic, and can use oxygen as a terminal electron acceptor in respiration. For example, in food microbiology it is important to be able to accurately identify food spoilage contaminants. Pin by Claudia Monsisvais-Ramos Qualified Unknown Bacteria Identification Bacteria Biochemical Test Flow Chart Microbiology Lab Unknown Bacteria Flow Chart Microbiology See more. I had a bacterial unknown of M. microbiology lab practical information part 1 Made with Explain Everything. Bacteria lies everywhere and affects multiple people therefore knowing the causes of the bacteria can help treat an ill patient. From 1984, the Bergey's Manual was renamed Bergey's Manual of Systematic Bacteriology is being published in separate volumes. Animal Pathogen City 7. The protocols are geared for microbiology educators at all levels and assume a basic understanding of general microbiology. October 10, 2007. Culture characteristic of bacteria 1. Kennedy (1) unless otherwise noted. Unknown Bacteria Dichotomous Key DAVID CHAINEY. • To demonstrate skill in coordinating the usual laboratory work with that of identifying an unknown organism. The microbes that are currently in the kingdom archaea was originally grouped with Bacteria. April 29, 2014. The identification of the unknown bacteria in this study was completed by using methods learned in the microbiology lab class for BIO203. It’s a gram negative but what bacteria is it?. Get an answer for 'Help to write a hypothesis in the introduction of an identification of two unknown bacterias in a microbiology lab report. Microbiology Anaerobic Gram-positive Non-sporeforming Rods and Anaerobic Gram-positive Cocci. These plasmids typically have a conserved backbone region and a variable accessory region that encodes host-beneficial traits. After the isolation of gram-­‐positive and gram-­‐negative bacteria from unknown A, specific biochemical tests were performed. A number of different variations are found, and the laboratory should standardise on one method. The micro lab website, your textbook, the web and assorted books available in lab will be the reference materials necessary for you to successfully complete the next several weeks of lab work. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. Bacteria that are gram negative will appear pink while those that are gram positive will appear purplish. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. MATERIALS AND METHODS:. OBJECTIVES • To learn specifically which diagnostic tests are required to identify a species of the Enterobacteriaceae family. Therefore, when working with bacteria you must wear a lab coat, safety glasses and gloves. Bacteria are classified and identified to distinguish one organism from another and to group similar organisms by criteria of interest to microbiologists or other scientists. Esculin hydrolysis was first described by Rochaix in 1924. Web Review of Todar's Online Textbook of Bacteriology. The MLG contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the. Choose from 500 different sets of microbiology lab exam identification unknowns flashcards on Quizlet. The data were analyzed with respect to the use of economical and practical procedures for the accurate identification of lactose-fermenting and -nonfermenting Enterobacteriaceae within 24 hr after isolation. The microbes that are currently in the kingdom archaea was originally grouped with Bacteria. 2016 Microbiology Laboratory Manual - Page 32 Microbiology Experiment #7 - Exoenzymes, Differential and Selective Media Every microorganism has different metabolic capabilities. " MATERIALS AND METHODS This is where the details of the study are listed. Escheria coli) I have to pick two tests to narrow down what I have. • Bacteria grow on solid media as colonies. Procedures were followed as stated in the lab manual and biochemical test handouts. The common light microscope used in the laboratory is called a compound microscope because it contains two types of lenses that function to. (1) Identification is key to know exactly how a particular bacteria should be treated or if treatment is necessary at all. The unknown bacteria will be one of the following: Enterococcus faecalis, Staphylococcus saprophyticus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Salmonella [I assume typhimurium], or Shigella [either flexneri or sonnei, we used both in our lab during the semester]. The procedures and sterile techniques learned throughout the course to identify bacteria were applied to these unknown. A major innovation in laboratory sciences occurred in the late 1960s, when manual miniaturized identification systems were first introduced into the clinical microbiology laboratory. If they show up + or - for a test then you know you have narrowed down the field some. Spring 2014 INTRODUCTION The purpose of this study was multifaceted: First, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment. One contains glucose; one contains lactose. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. Report on Identification of Mixed Culture Unknown Lab Exercise 33. General Microbiology 203 INTRODUCTION. Also, it should also be kept in mind, that while shape is a characteristic of bacteria, some bacteria posses the ability to change shape; thus shape shouldn't be strictly relied upon for identifying the unknown bacteria. Remember, each member of the group must: 1. In this lab, students prepare and analyze a virtual bacterial DNA sample. In lab, we have to identify two unknown organisms in a sample of nutrient broth. Because both the presence and type of bacteria that may be in the food is unknown, BSL2 containment practices should be used throughout the entire procedure. After a successful Gram stain it was classified as a Gram positive cocci which narrowed it down to three bacteria; Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. Microbiology Lab Cyanobacteria, Protozoans, and Algae Prokaryotic and Eukaryotic Cells As you know, the building blocks of life are cells. Identification of an Unknown Bacterium and Writing Up a Report Start studying Microbiology Dichotomous Key Quiz. Microbiology Unknown Identification Chart 25 Awesome Bacteria Test microbiology unknown identification chart 25 awesome bacteria test dailyrevshare. Identification of unknown bacteria using dichotomous key-Part 1 Microbiology: Bacteria Identification Flowchart of Facultative Anaerobes - Duration: 15:06. Is s aureus a mesophile - answers. MICROBIOLOGY MODULE Methods of Isolation of Bacteria Microbiology 112 Notes properties (Gram positive: purple; Gram negative: pink/red). The manual include four divisions of the kingdom Prokaryotae. We use phenotype analysis to identify bacteria. Results from these susceptibility tests usually take 24-48 hours, though some can take weeks. MATERIALS AND METHODS. Access to the laboratory is limited or restricted at the discretion of. Tyler Wolfangel. Bacteria that are gram negative will appear pink while those that are gram positive will appear purplish. Recently, mobile tet (X) variants were reported in diverse pathogens from different sources. Note: this will require time in addition to your regular lab period to complete. Unknown P was successfully identified as Staphylococcus aureus. LAB STUDY GUIDE (for lab quizzes and lab practicals). id of unknown bacteria laboratory videos ID OF UNKNOWN BACTERIA LABORATORY IMAGES The images linked here directly relate to the lab exercise and are designed to help you review and study that lab material while outside of lab. Subjects: Microbiology which is then used to identify the bacterium from the Enteropluri __4__. , University of Oregon Science Education in Rural Schools Research Interests: Ecology, evolution and systems biology of Pelagibacterales (SAR11). I went through a wet lab in school, and starting off with gram+ or gram- narrows down the field alot. and observe the. Indole test: This test is used to determine the ability of an organism to split tryptophan to form the compound indole. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. Many species of non-fermenting Gram-negative bacilli (non-fermenters) are important opportunistic and nosocomial pathogens. plate or tube inoculations on the first lab session in the week. The coagulase-positive staphylococci constitute the most pathogenic species S aureus. Examine the slide under a microscope at 1000X magnification. Lab12-Medical Microbiology- Part1- Differential Media/Biochemical Tests, Sp2012 (Set all of your margins to ½") Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. Oxidase Test. lab 3: obtaining pure cultures from a mixed population; lab 4: enumeration of microorganisms; lab 5: direct stain and indirect stain; lab 6: gram stain and capsule stain; lab 7: endospore stain and bacterial motility; lab 8: using biochemical testing to identify bacteria; lab 9: fungi, part 1 - the yeasts. Bacteria can communicate with each other and coordinate their actions. Late reports will be penalized by 20 percent for each day late. Identification of gram-negative aerobes relies on whether or not. I have a test in my Microbio Lab tomorrow. The biochemistry of Archaea and Bacteria is a significant parameter in their classification. From heart valve Sound and Fury in the Microbiology Lab. Key features of these bacterial colonies serve as an important criteria for their identification. The carbohydrate fermentation test is used to determine whether or not a bacteria can utilize a certain carbohydrate. Isolation and Identification of Gram (+) Organism #13 – Bacillus subtilis 4. 2) Enzymatic Reactions 1. Our main functions are: specialized services to identify diseases other labs may not be able to detect or diagnose. The identification of lactose-fermenting Gram-negative rods belonging to the bacterial family Enterobacteriaceae (bacteria commonly referred to as coliforms) in water is often used to determine if water has been fecally contaminated and, therefore, may contain disease-causing pathogens transmitted by the fecal-oral route. If the doctor can then identify which microorganism it is, then he will be. The following study was performed using all of the methods used in general microbiology laboratory class. Materials and Methods. Unknown labeled 104 was given to me in lab by the instructor. Report for "Unknown" Identification Project Jessica Olivares Francisco Alarcon-Chaidez 12/02/2015 Negative and Positive Identification Of Unknown Bacterial Strains Report Introduction/Purpose The objective of this lab was to classify the unknown bacterial strains provided utilizing a number of biochemical procedures. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in. The Lab Exercise Instructions Part I provide a thorough explanation of streak plating and how to prepare bacterial smears for differential staining. Next the bacteria are cultured and put through biochemical tests to determine their identification. This medium is important in medical laboratories as one method of distinguishing pathogenic microbes in a short period of time. Expensive equipment and materials are not usually necessary for recovery of common aerobic or facultatively anaerobic bacterial pathogens, such as Staphylococcus spp, Streptococcus spp, and coliforms. Pin by Claudia Monsisvais-Ramos Qualified Unknown Bacteria Identification Bacteria Biochemical Test Flow Chart Microbiology Lab Unknown Bacteria Flow Chart Microbiology See more. US East Coast: 800-220-3675 US West Coast: 866-798-1089 Canada: 888-831-0722. ___1___ usually from Gram Positive Bacteria cause. In order to identify these bacteria, a series of different laboratory tests must be done. 29 April 2014. The MLG contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the. A major innovation in laboratory sciences occurred in the late 1960s, when manual miniaturized identification systems were first introduced into the clinical microbiology laboratory. Unknown C was determined to be a Gram- negative rod. The microscope is a very important tool in microbiology, but there are limitations when it comes to using one to observe cells in general and bacterial cells in particular. Identifying Unknown Bacteria Lab 1. Methods could also be referred to the lab manual pages 159-163. Samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms. Microbiology Unknown Identification Chart 25 Awesome Bacteria Test microbiology unknown identification chart 25 awesome bacteria test dailyrevshare. (9) In 1970, Facklam and Moody determined that the use of the bile esculin test was a reliable way of identifying group D streptococci from non-group D streptococci. Example of Unknown Lab Report, Microbiology. pdf] - Read File Online - Report Abuse. Learn vocabulary, terms, and more with flashcards, games, and other study tools. bronchiseptica, B. The first procedure that was done was a gram stain followed by a streak of the unknown on a TTS plate in order to determine the gram reaction and observe the colony. 5 m M MgCl 2. Carbohydrate Utilization. Cells are often stained to enhance cellular features, and the properties of the cell wall are used in the classification of microorganisms. Microbiology Laboratory Skills Test: Bacterial Unknown ?•. Identifying Unknown Bacteria Lab 1. General Microbiology Concordia University, Irvine February 24, 2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop. Our services include the analysis of fungi (), bacteria (legionella, e. Lab #7-8: Identification of unknown bacteria in mixed cultures I. Overview of a medical microbiology laboratory - Duration: 7:55. The cells were not joined together, but all of them were single rods. Numbers of different methods were used to identify the specific bacteria given. Floyd and Dr. Prokaryotes are the dominant living creatures on Earth, having been present for perhaps three-quarters of Earth history and having adapted to almost all available ecological habitats. December 3, 2013 Introduction. However, many studies have demonstrated the impact of antimicrobial stewardship on patient outcomes. Not long ago, DNA sequencing was a time-consuming, tedious process. This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor. Differential Staining Techniques Viewing Bacterial Cells. Our services include the analysis of fungi ( mold ), bacteria ( Legionella, E. Escheria coli) I have to pick two tests to narrow down what I have. Prerequisite(s): Concurrent internship in affiliated hospital and all degree requirements for BS in microbiology except 30 hours clinical. Gram-staining was firstly introduced by Cristian Gram in 1883. EMSL Test Code: M192. hinzii and B. We work with public health partners in Canada and abroad to prevent the spread of infectious diseases. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Essay Microbiology Unknown Bacteria. UNKNOWN LAB REPORT. Identification of unknown bacteria using dichotomous key Microbiology: Bacteria Identification Flowchart of. Transmission E. microbiology lab practical information part 1 Kim Owen. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. A major innovation in laboratory sciences occurred in the late 1960s, when manual miniaturized identification systems were first introduced into the clinical microbiology laboratory. Introduction Special Safety Precautions: All bacterial cultures are potential pathogens. The tests done include: Culturing and Gram staining for Morphology. College of health sciences-HMU Lab 6 2. Step 2: Identification. Bacteria do not come in pure cultures (except in classroom exercises). The data were analyzed with respect to the use of economical and practical procedures for the accurate identification of lactose-fermenting and -nonfermenting Enterobacteriaceae within 24 hr after isolation. In this lab, students identify an unknown bacteria using a biochemical method and a molecular method. TTC becomes red when it is reduced by these bacteria. Bachman, William D. Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. CHAPTER 7 Identification and Characterization of Neisseria meningitidis. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. In this test, researchers grow the unknown bacteria in a petri dish, then introduce bacteriophage, a special kind of virus. Identifying Unknown Bacteria Lab 1. • A colony is defined as a visible mass of microorganisms all originating from a single mother cell. To identify unknown bacteria aseptic techniques were followed per the McDonald et al. Reference. UNKNOWN LAB REPORT. The unknown bacterium could possibly be one of seven different bacteria that we have worked with during our laboratory course. You may want to run some of the following tests. A bright-field microscope was used on 1000X magnification. After the isolation of gram-­‐positive and gram-­‐negative bacteria from unknown A, specific biochemical tests were performed. Unknown C was determined to be a Gram- negative rod. Microbiology Laboratory: Test: Identification of Unknown Bacteria and Fungi by DNA Sequencing: Description: EMSL can identify your unknown, pure, fungal or bacterial isolates using PCR and DNA sequencing. The appearance of the discrete masses of growth or colonies that can be grown from isolated bacteria on the surface of the solid medium (nutrient agar) can be used to study the size of the colonies (diameter in mm), their outline (whether circular, entire, indented or wavy or. Examples Of Salt Tolerant Bacteria. These include vancomycin, colistin, and nystatin. In this lab, students prepare and analyze a virtual bacterial DNA sample. The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity. Article Summary: In a microbiology lab it's essential to avoid contamination of sterile materials and isolated cultures. Microbiology News. coli in microbiology laboratory. All procedure performed were done so according to the microbiology laboratory manual. Approximately 30% of adults and most children are healthy. Laboratory Tests. Therefore Proteus vulgaris was indeed one of the unknown bacteria. The microbiology gold standard is the culture of a sterile body fluid such as CSF or blood. Because of this variety, bacteria may be grouped using many different typing schemes. UNKNOWN LAB REPORT. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Unknown C was determined to be a Gram- negative rod. This experiment of finding the unknown bacteria of 112 was first started by inoculating a nutrient agar (NA) plate. Observe the shape of the bacteria. Spore of Clostridium botulinum. Start studying Identification of Unknown bacteria. 13 Viruses, Viroids, and Prions. Morphology and. It is in PDF format, if you do not have Acrobat Reader please go to www. Laboratory Exercises in Microbiology, 5th ed. This lab was designed for 500 students split into lab sections of 20. , Newark, Del. See page 84 of the Difco/BBL Manual. All spills must be reported immediately. This medium is important in medical laboratories as one method of distinguishing pathogenic microbes in a short period of time. It is a common medium used to culture bacteria because: It is a great enrichment medium for fastidious bacteria. Detects production of tryptophan producing indol Blue Green Tests for utilization of citrate as carbon source Pink Wheat / no color Measures the production of change urease Black precipitates Wheat / no color Tests for the production of change iron 2 sulfide (FeS) Motile / Non-motile / little to Tests whether bacteria is movement no movement. lab 3: obtaining pure cultures from a mixed population; lab 4: enumeration of microorganisms; lab 5: direct stain and indirect stain; lab 6: gram stain and capsule stain; lab 7: endospore stain and bacterial motility; lab 8: using biochemical testing to identify bacteria; lab 9: fungi, part 1 - the yeasts. From heart valve Sound and Fury in the Microbiology Lab. MICROBIOLOGY FALL 2012. microbiology flowchart unknown bacteria. carbohydrate utilization tests (sugar test). Creating a dichotomous key: Phenetic identification of bacteria-Part 2 How to identify an unknown bacteria - Duration: bacterial identification methods in the microbiology laboratory. Introduction to Clinical Microbiology. Salmonella serotyping is a subtyping method of identification based on the identification of distinct cell wall, flagellar, and capsular antigens with known antiserum, as will be discussed in Lab 17. Being able to culture, isolate and identify a unknown bacteria is one of the corner stones in microbiology BIO 203, as well as the ability to use different techniques to distinguish and identify different bacteria. of winning Nobel Prize (LOL) Serious Titles: Identification of unknown bacteria: Materials, mehtods and results. Identification of unknown bacteria using dichotomous key-Part 1 microbiology lab practical information part Kim Owen 226,115 views. Explore clinical microbiology techniques, such as differential staining, differential and selective media and biochemical assays, then use your new skills to identify the bacteria. Identification of an Unknown Bacterium and Writing Up a Report Start studying Microbiology Dichotomous Key Quiz. I had my times of mistakes trying to find out these bacteria from the rest but I stuck to the fundamental basics of the lab manual and believed in myself. Microbiology. There are two genera of bacteria that can appear as a streptococcus arrangement that we will take up in the lab: the genus Streptococcus and the genus Enterococcu s. identification texts, to deduce the Genus and Species designation of the unknown organism provided. Identify and describe culture media for the growth of bacteria, including examples of all-purpose media, enriched, selective, differential, defined, and enrichment media. Students are introduced to the Acid-fast and Endospore stains. Identification of bacteria is a part of the diagnosis. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. To identify bacteria through DNA analysis, researchers can measure the ratio of guanine-cytosine pairs. By comparing these capabilities, one can group and identify the organisms being studied. com) Gram-positive; Gram-negative. The anaerobic Gram-positive cocci and anaerobic Gram-positive non-sporeforming rods such as Anaerobic+bacteria, Bifidobacterium, Eubacterium, Lactobacillus and Propionibacterium species are, for the most part, components of the normal flora of the mucosal surfaces and, to a lesser extent, the skin. The goal of this lab report is 1) to demonstrate comprehension of the methods and lab techniques learned during the semester 2) to explain the tests performed on each isolated unknown that led to the identification of each unknown 3) and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns. 4) Related Notes: Share the article on:Biochemical Test of Bacillus subtilis ­Basic Characteristics Properties (Bacillus subtilis) Catalase Positive (+ve) Citrate Positive (+ve) Flagella Flagellated Gas Negative (-ve) Gelatin Hydrolysis. "Identification of bacteria using two degenerate 16s rDNA sequencing primers" by Boye, et al. After a series of differential tests, a chart of bacteria was referenced to discover the unknown bacteria was E. It distinguishes between hemolytic and non-hemolytic bacteria. Kennedy (1) unless otherwise noted. Spring 2014 INTRODUCTION The purpose of this study was multifaceted: First, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Each test performed, provided us with some key information about the unknown organism in question and how the bacteria. Identification of gram-negative aerobes relies on whether or not. Spring 2014 INTRODUCTION The purpose of this study was multifaceted: First, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment. Dec 16, 2019 - Qualified Unknown Bacteria Identification Bacteria Biochemical Test Flow Chart Microbiology Lab Unknown Bacteria Flow Chart MicrobiologyBack To 18 Memorable Unknown Bacteria IdentificationExpert Unknown Bacteria Identification…. Often used to differentiate species from the genera Clostridium and Bacillus. MATERIALS AND METHODS:. Media used for identification of bacteria by testing various biochemical reactions are sorted alphabetically and by type of test. There is an online GLOSSARY OF TERMS, linked to terms within the text of the lab exercises. Answer to Identify the unknown. In this test, researchers grow the unknown bacteria in a petri dish, then introduce bacteriophage, a special kind of virus. Materials and Methods A mixed culture of two unknown bacteria was provided by the instructor. bronchiseptica, B. Price is per isolate. Start studying Microbiology Ch. The following study was performed using all of the methods used in general microbiology laboratory class. Kateryna Petrakova. Lab 7 will demonstrate that different bacteria, because of their unique enzymes, are capable of different biochemical reactions. The results of these tests on the suspected microorganism are then compared to known results for that organism to confirm its identification. pdf] - Read File Online - Report Abuse. b) pathogens that are no longer alive in the patient sample, c) species that cannot be differentiated by conventional testing. Principle: The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. In microbiology laboratory class many techniques were studied to learn how a microorganism will react in certain conditions. Explain a rapid biochemical test for the identification of Staphylococcus aureus? Explain the principle of nitrate reduction test?. Microbiology 20 Biochemical Unknown – Spring 2009 (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May 14th Biochemical tests in microbiology lab. 13 Viruses, Viroids, and Prions. One contains glucose; one contains lactose. LAB STUDY GUIDE (for lab quizzes and lab practicals). Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. Doing specific tests on the two bacteria's will give you the ultimate answer at the end as to what the bacteria is, so correct measures can be taken. Out of a possible 70 points you will be graded as follows: Successfully performing the necessary tests to identify your unknown (25 pts):. UNKNOWN LAB REPORT. The lab exercises you cover while working on your unknown will be on your lab practical, and your lab practicals count for much more of your lab grade than your unknown does. For example, if a patient has an infection, the doctor can isolate the microorganism causing the illness. Numbers of different methods were used to identify the specific bacteria given. Why Microbiology Identification of Bacteria? In this section you can learn and practice Microbiology Questions based on "Identification of Bacteria" and improve your skills in order to face the interview, competitive examination and various entrance test (CAT, GATE, GRE, MAT, Bank Exam, Railway Exam etc. Staphylococcus aureus (coagulase-positive staphylococci) Staphylococcus aureus is the most pathogenic species and is implicated in a variety of infections. Unknown P was successfully identified as Staphylococcus aureus. Josephine, Mizer E. 2012 – Although growth was best on the TSA plate, the Gram(+) organism was unable to be. Discussion/Conclusion. Explore clinical microbiology techniques, such as differential staining, differential and selective media and biochemical assays, then use your new skills to identify the bacteria. Research Methodology. Learn vocabulary, terms, and more with flashcards, games, and other study tools. INTRODUCTION. It is called "Morphological Unknown. 5 m M MgCl 2. Unlike a virus, bacteria are easily traceable by simple staining methods. Describe examples of biosynthesis products within a cell that can be detected to identify bacteria Accurate identification of bacterial isolates is essential in a clinical microbiology laboratory because the results often inform decisions about treatment that directly affect patient outcomes. See page 84 of the Difco/BBL Manual. UNKNOWN LAB REPORT. MR (Methyl Red) Nitrate Reduction. you will apply an expanding array of microbiology laboratory skills to grow and investigate the colonial, cellular, and metabolic properties of one of the bacterial species from your skin culture. If unable to collect 3 mL of urine, collect in sterile specimen container (37777) or yellow top tube (104184) and transport urine specimens to the Microbiology Laboratory or refrigerate within 30 minutes. Identification of Bacteria: Step # 1. of Bacillus megaterium. Step 2: Identification. You will conduct tests appropriate for your organism to determine genus and species identification. Culture media in microbiology is a media where microorganisms like bacteria from a sample is grown into colony for the identification of particular organism. Learn microbiology lab exam identification unknowns with free interactive flashcards. We also offer a range of biochemical reagents, known for the identification of specific metabolisms and to differentiate between bacteria. However, the genomes of numerous pathogens have already been sequenced, particularly by The Institute for Genomic Research, TIGR, in Rockville, MD, and the Sanger Centre in Hinxton, UK, and there is an increasing economic interest in sequencing microbial genomes not only from pathogens but also from other, as yet unknown bacteria. Antigenic Characters 5. Microbes technology Question and answer Unknown Bacteria Lab Report- Microbio Preview text Experiment 1 BIOC1010: Introduction to Microbiology Laboratory report: experiment 1 Culture medium preparation Introduction Briefly describe what a culture medium is, why it needs to be sterile and what the aims of the experiment were. No one will receive a final grade in Microbiology Lab until the entire lab area is clean (beer refrigerator, lab counters, incubators, etc). Second, utilizing those techniques allowed for the identification of two unknown bacteria. • A lab practical (20 points) requires each student to. Therefore, they must be identified and susceptibility testing must be performed so the treating physician will know what is causing the problem and how best to treat it. To introduce and demonstrate various steps involved in the identification of a bacterial unknowns from a mixed culture of organism. IDENTIFICATION OF UNKNOWN BACTERIA LAB REPORT Grace K. Gram positive did not grow. Unknown bacteria study: Experimental design, results, and discussion. Step 2: Identification. "Identification of bacteria using two degenerate 16s rDNA sequencing primers" by Boye, et al. This identification was reached by only one biochemical test and close observation. Unknown Number 115. In response to a hostile environment, some bacteria adopt a dormant state by generating a spore, easily visualized with the light microscope. Samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms. ), and 16S rRNA gene sequence. Bacteria that are gram negative will appear pink while those that are gram positive will appear purplish. Methods could also be referred to the lab manual pages 159-163. Many pathogenic bacteria exhibit a relatively narrow range of pH over which they will grow. Identify the person who, around 350 Discuss at least two reasons why the gram stain is one of the most important stains used in the clinical microbiology lab of a hospital. Identification of Unknown Bacteria Identify unknown bacteria. Kimsey Cooper. Identification of unknown bacteria by. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The cells in your sample will be taken to a lab and put in a special environment in a lab to encourage cell growth. Microscopy to identify cell shape or appearance of spores. Gram staining is a procedure that allows you to divide bacteria into 2 common types: Gram positive, and Gram negative. The decision to identify the coryneform bacteria to the species level is recommended when the bacteria are cultured from normally sterile sites, such as blood (two or more positive blood cultures, except when recovered from the same set) or cerebrospinal fluid (CSF), if the bacteria appear in adequately collected clinical material as the. The microscope is a very important tool in microbiology, but there are limitations when it comes to using one to observe cells in general and bacterial cells in particular. A total of 5,137 enterobacterial clinical isolates were examined with three series of biochemical tests. hinzii and B. microbiology laboratory class for the identification of an unknown bacterium. The identification of lactose-fermenting Gram-negative rods belonging to the bacterial family Enterobacteriaceae (bacteria commonly referred to as coliforms) in water is often used to determine if water has been fecally contaminated and, therefore, may contain disease-causing pathogens transmitted by the fecal-oral route. Learn microbiology lab identification unknowns with free interactive flashcards. Microbiology Dichotomous Key Quiz Flashcards | Quizlet Dichotomous Key. carbohydrate utilization tests (sugar test). This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. The lecture will: 1. Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. The study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. Click to close image, click and drag to move. It has been a widely used reference since the publication of the first edition in 1923. Microorganisms sense and adapt to changes in their environment. I have a test in my Microbio Lab tomorrow. Report and Guidelines. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. Your ability to make aseptic transfers, perform the. The biochemistry of Archaea and Bacteria is a significant parameter in their classification. For EU (identification of a bacterial species belonging to the Enterobacteriaceae family) and OU (identification of a non-Enterobacteriaceae bacterial species), we use the conventional microbiological diagnostic techniques to identify the species. id of unknown bacteria laboratory videos ID OF UNKNOWN BACTERIA LABORATORY IMAGES The images linked here directly relate to the lab exercise and are designed to help you review and study that lab material while outside of lab. coli, salmonella, listeria, etc), mycotoxins, endotoxins, allergens, pollen and particulates in air, swab, water, soil, bulk, dust, wipe, food and consumer products. Enter the Lab. In this test, researchers grow the unknown bacteria in a petri dish, then introduce bacteriophage, a special kind of virus. Lansing, Harley P. An unknown labeled 123 was given out by the lab instructor. MATERIALS AND METHODS. It is important to classify bacteria in order to identify them. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. you are here->home->Biotechnology and Biomedical Engineering->Microbiology Virtual Lab II->Isolation and Identification of Two Bacterial Unknowns. The procedures taken to figure out, and isolate the two separate bacteria were taught thus far in Microbiology lab class. Lab Exercise #3b - ID of Unknown Bacteria 3 characteristics of these cells is needed to identify the genus and species of the bacterium; the metabolic capabilities of the organism are studied in more detail. The results of the biochemical tests along with deductive reasoning and elimination led to the identification the unknown bacteria. Microbiology Unknown Lab Report Essay 2396 Words | 10 Pages. MR (Methyl Red) Nitrate Reduction. 0 µm in diameter, typically occurring in pairs and chains of varying length when grown in a liquid medium , and often occurring singly, in pairs. However, there are a few cocci shaped gram negative bacteria as well.